Guide Advances in Salivary Diagnostics

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Protein detection and quantitation technologies for gel-based proteome analysis. Methods Mol Biol.

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Current two-dimensional electrophoresis technology for proteomics. Instruments and methods in proteomics. Mahmood T, Yang PC. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Aebersold R, Mann M. Mass spectrometry-based proteomics. Nguyen S, Fenn JB.

Gas-phase ions of solute species from charged droplets of solutions. Chait BT. Mass spectrometry in the postgenomic era. Annu Rev Biochem. Automated protein identification by tandem mass spectrometry: issues and strategies. Matrix-assisted laser desorption ionization-time of flight maldi-tof mass spectrometry for detection of antibiotic resistance mechanisms: from research to routine diagnosis. Clin Microbiol Rev. Probability-based protein identification by searching sequence databases using mass spectrometry data.

Niessen WM, Sn. Encyclopedia of Spectroscopy and Spectrometry.

Elsevier; Cottrell JS. J Proteomics. Zhang W, Zhao X. Method for rapid protein identification in a large database.

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BioMed Res Int. A proteomics search algorithm specifically designed for high-resolution tandem mass spectra. Rapid identification of proteins by peptide-mass fingerprinting. Curr Biol. An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.

J Am Soc Mass Spectrom. The Human Protein Atlas.

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Downloadable data. Tissue-based map of the human proteome. A dynamic range compression and three-dimensional peptide fractionation analysis platform expands proteome coverage and the diagnostic potential of whole saliva.

Delta Dental Salivary Diagnostics

One decade of salivary proteomics: current approaches and outstanding challenges. Clin Biochem. Identification of human whole saliva protein components using proteomics. Frenkel ES, Ribbeck K. Salivary mucins in host defense and disease prevention. J Oral Microbiol. Mucins, mucus, and goblet cells. Bennick A. Salivary proline-rich proteins. Mol Cell Biochem. Histatins, a novel family of histidine-rich proteins in human parotid secretion. J Biol Chem. Evidence of intact histatins in the in vivo acquired enamel pellicle. J Dent Res.

Saliva in the diagnosis of diseases | International Journal of Oral Science

In vitro identification of histatin 5 salivary complexes. PLoS One. Identification and characterization of histatin 1 salivary complexes by using mass spectrometry. Comparative human salivary and plasma proteomes. Salivary amylase - The enzyme of unspecialized euryphagous animals. Arch Oral Biol. Revealing the amylase interactome in whole saliva using proteomic approaches. The clinical application of proteomics. Clin Chim Acta. Salivary secretion: mechanism and neural regulation. Monogr Oral Sci.

Current developments in salivary diagnostics

Wong D. Salivary diagnostics. Ames: Wiley-Blackwell; Potential applications of human saliva as diagnostic fluid. Acta Otorhinolaryngol Ital. Salivary proteomics for oral cancer biomarker discovery.

Clin Cancer Res. Diagnostic model of saliva protein finger print analysis of patients with gastric cancer. World J Gastroenterol. Initial comparison of proteomic profiles of whole unstimulated saliva obtained from generalized aggressive periodontitis patients and healthy control subjects. J Periodontal Res. Systematic comparison of the human saliva and plasma proteomes. Quantitative proteomics reveals myosin and actin as promising saliva biomarkers for distinguishing pre-malignant and malignant oral lesions.

Potential biomarkers in saliva for oral squamous cell carcinoma. Oral Oncol. Comparative proteomic analysis of whole saliva from chronic periodontitis patients. Xiao H, Wong DT. Proteomics and its applications for biomarker discovery in human saliva. Proteomic analysis of human saliva from lung cancer patients using two-dimensional difference gel electrophoresis and mass spectrometry.

Several integrated tools streamline common tasks, such as converting between ID formats and retrieving sequences. The combination of a wide variety of databases, an easy-to-use interface, robust programmatic access and the array of tools make Central Portal a one-stop shop for biological data querying.

SDxMart is a BioMart data portal that hosts salivary proteomic, trans-criptomic, metabolomic and microRNA data and offers access to the data by using the BioMart interface and querying environment. SDxMart is designed to provide a variety of queries to facilitate saliva biomarker discovery including complex queries that integrate genomic, clinical and functional information. The SDxMart holds data from projects of oral diseases and systemic diseases including oral cancer, Sjogren's syndrome, pancreatic cancer and breast cancer.

The types of datasets are: i proteomics; ii trans-criptomics; iii microRNA; and iv metabolomics. In addition, the SDxMart is imported with several public databases including Ensembl genome database Ensembl release 37 ,20 and the number of resources is continuously growing.

The SKB is being created to facilitate researchers using salivary data from multiple perspectives.